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Pasqual,Moacir; Dutra,Leonardo Ferreira; Araujo,Aparecida Gomes de; Figueiredo,Milene Alves de. |
The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Nutrient medium; Breeding. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000500029 |
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